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In our case, in addition to a binding threshold for all mutated peptides, we distinguished two groups regarding the binding capability of the WT peptide. In the primary group , the place WT peptides had been poor or null binders, an important proportion of peptides contained the mutation at HLA-anchoring residues (e.g. peptides CAM69-6, CAM73-9). However, in the second group all peptides contained the mutation at non-anchor residues.
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